By integrating manipulation by light, manipulation of light, and artificial intelligence, we aim to establish a new generation of intelligent optical measurement technologies. Combining conventional optical microscopy with optical rotation and machine learning, we achieve high-resolution volumetric imaging and accurate classification of organisms. Our mechanoscopy platforms, including adhesion frequency assays, quantify dynamic cell-cell interactions, cell-substrate adhesion, and receptor-ligand binding forces in biocompatible environments with well-controlled geometry, while revealing their relationships with biochemical organization and signaling. In parallel, we develop chiroptical spectroscopy systems that enable label-free, highly sensitive enantiodiscrimination.